Table 1 Sequences of primers and probes for real-time RT-PCR
From: MiR-183-5p promotes migration and invasion of prostate cancer by targeting TET1
Gene | Primer / Probe | Sequence (5’ to 3’) |
|---|---|---|
miR-183-5p | Forward | CCTGTTCTGTGTATGGCACTGGT |
Reverse | TTCACTGACTGAGACTGTTCACAGTG | |
Human TET1 | Forward | CCATCTGTTGTTGTGCCTCTG |
| Â | Reverse | GCCATTTACTGGTTTGTTGTCA |
| Â | Probe | FAM-AGGTTATAAAGGAAAACAAGAGGCCCC-BHQ-1 |
Human GAPDH | Forward | CAACTACATGGTTTACATGTTC |
Reverse | GCCAGTGGACTCCACGAC | |
Probe | CY5-TTTGGTCGTATTGGGCGCCTG-BHQ-1 |