Fig. 1From: Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancerCSCs and CSCs-exo were identified. The displayed results were obtained from T24 cell line. AÂ The markers of CSCs, CD105 and Nestin, were examined by immunofluorescence assay. BÂ The morphology of CSCs-exo was observed under transmission electron microscope. CÂ Sizes of CSCs-exo were analyzed by nanoparticle tracking analysis. DÂ The biomarkers of CSCs-exo, TSG101, CD63 and CD9, were examined by western blot assay. Calnexin was used as a negative marker for CSCs-exo. CSC Cancer stem cells, BCSC Bladder cancer stem cells, CSCs-exo Exosomes derived from cancer stem cellsBack to article page