Fig. 4

GPR12 was the target of miR-105-5p. A The potential targets of miR-105-5p were predicted by miRDB (http://mirdb.org/), miRWalk (http://mirwalk.umm.uni-heidelberg.de/) and TargetScan (http://www.targetscan.org/vert_71/), and the results were expressed as the Venn diagram. B mRNA expressions of GPR12, ITM2B, ZNF24, and NSL1 were detected after miR-105-5p mimic was transfected into T24 cells. C Sequences of putative binding sites of miR-105-5p and GPR12 were listed, and the mutant binding sequence of GPR12 was designed. D Luciferase reporter assay was performed to validate the binding between miR-105-5p and GPR12. Experiments were conducted thrice for each approach. ^*P < 0.05; ^***P < 0.001