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Fig. 1 | BMC Urology

Fig. 1

From: Sakuranin represses the malignant biological behaviors of human bladder cancer cells by triggering autophagy via activating the p53/mTOR pathway

Fig. 1

Sakuranin suppressed T24 cell proliferation and accelerated apoptosis. A: Molecular structure of Sakuranin; B: T24 cells were treated with Sakuranin at concentrations of 0, 1.25, 2.5, 5.0, 10, 20, 40, and 80 mg/mL for 24, 48, and 72 h. Cell viability was detected by CCK-8 assay; C-E: The 24-, 48- and 72-h IC50 were analyzed by GraphPad Prism 8.0; F: Effect of Sakuranin at 48-h IC50 concentration (6.8 mg/mL) on cell viability was assessed by CCK-8 assay; G: Cell proliferation was assessed by EdU assay; H: Cell colony formation area was examined using plate clone formation assay; I: Cell apoptosis was assessed by flow cytometry. Data were expressed as mean ± SD, and three technical repetitions of cell experiments were conducted. Comparisons between two groups were performed by independent t-test. ***p < 0.001

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BMC Urology

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