Fig. 1

Identification of urinary bladder mast cells. (A) Shows identification of live cells (LC) through the exclusion of propidium iodide, followed by forward scatter (FSC) and side scatter (SSC) doublet discrimination and the identification of CD45.2⁺ cells. (B) Shows FMOCs for FcεRIα and CD117 antibodies, which were used to assist the identification urinary bladder mast cells. (C) Shows the frequency of LC/FSC Singlet/SSC Singlet CD45.2⁺ cells and (D) shows mast cell frequency within CD45.2⁺ populations