Table 1 The primer sequences used in this study
From: CircRNA/miRNA/mRNA axis participates in the progression of partial bladder outlet obstruction
Primer name | Sequence (5′-3′) |
|---|---|
R-Gata4-F | CTGCGAGACACCCCAATCTC |
R-Gata4-R | GCCGGTTGATACCATTCATCTTG |
R-Pigr-F | TGCTGGACTGTATGTTTGCC |
R-Pigr-R | GCATCATTTGCCACTTCACG |
R-Diaph3-F | CCGGGTGCCTTATGAGAAAATC |
R-Diaph3-R | TGCTCATCACGACAGCAAAC |
rno-miR-30c-1-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGAGTA |
rno-miR-30c-1-3p-F | CTGGGAGAGGGTTGTTTA |
rno-miR-153-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCTGC |
rno-miR-153-5p-F | GTCATTTTTGTGATGTTGC |
rno-miR-135b-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAT |
rno-miR-135b-5p-F | TATGGCTTTTCATTCCTAT |
Universe-R | GTGCAGGGTCCGAGGT |
R-chr3:113195876|113197193-F | TGGTTCACTCTCTGACGTCT |
R-chr3:113195876|113197193-R | TGCAAAGGTGGCTGATGACA |
R-chr5:122655270|122671094-F | AGGAAATCATGGTAGGCTCCA |
R-chr5:122655270|122671094-R | GCATTCAGAAGAAAAGCAAGTCAG |
R-chr1:126188351|126195625-F | TCCTTTTAGCACATCAGAAATCACT |
R-chr1:126188351|126195625-R | GCTCCCATGACAGGCCTC |
Rat-GAPDH-F | GCAAGAGAGAGGCCCTCAG |
Rat-GAPDH-R | TGTGAGGGAGATGCTCAGTG |
Rat-U6-F | CTCGCTTCGGCAGCACA |
Rat-U6-R | AACGCTTCACGAATTTGCGT |