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Influence of TPH2 and HTR1A polymorphisms on lifelong premature ejaculation risk among the chinese Han population

BMC Urology volume 23, Article number: 86 (2023) Cite this article

Abstract

Background

Lifelong premature ejaculation (LPE) is one of the most common ejaculatory dysfunctions in men. The serotonin (5-HT) synthesis rate-limiting enzyme (TPH2) and receptor (HTR1A) in the 5-HT regulatory system may play a key role in the pathogenesis of LPE. However, there are few studies on the effects of TPH2 and HTR1A polymorphisms on LPE risk. We speculated that TPH2 and HTR1A polymorphisms may affect the occurrence and development of LPE in the Chinese Han population.

Methods

In this study, 91 patients with LPE and 362 normal controls aged 18 to 64 years were enrolled in the male urology department of Hainan General Hospital in China from January 2016 to December 2018. The SNPs in HTR1A and TPH2, which are related to 5-HT regulation, were selected as indexes to genotype the collected blood samples of participants. Logistic regression was used to analyze the correlation between SNPs of HTR1A and TPH2 with LPE susceptibility, as well as the relationship with leptin, 5-HT and folic acid levels.

Results

The results revealed that HTR1A-rs6295 increased LPE risk in recessive model. Rs11178996 in TPH2 significantly reduced susceptibility to LPE in allelic (odds ratio (OR) = 0.68, 95% confidence interval (95% CI) = 0.49–0.96, p = 0.027), codominant (OR = 0.58, 95% CI = 0.35–0.98, p = 0.040), dominant (OR = 0.58, 95% CI = 0.36–0.92, p = 0.020), and additive (OR = 0.71, 95% CI = 0.52–0.98, p = 0.039) models. Grs11179041Trs10879352 could reduce the risk of LPE (OR = 0.44, 95% CI = 0.22–0.90, p = 0.024) by haplotype analysis.

Conclusion

HTR1A-rs6295 and TPH2-rs11178996 are associated with LPE risk in the Chinese Han population based on the finding of this study.

Peer Review reports

Introduction

Premature ejaculation (PE) is one of the most common ejaculatory dysfunctions in males, and approximately 20 − 30% of males have experienced PE. PE seriously affects not only the physical and mental health of patients but also sexual partners, marital relations, and family stability [1, 2]. The onset of PE is not age-specific, and PE may occur in a large range of people from 18 to 64 years. PE can be divided into two types according to the nature and the time of onset, that is lifelong premature ejaculation (LPE) and acquired premature ejaculation (APE) [3, 4]. On the basis of the last international society for sexual medicine (ISSM) for the definition of PE: [1] ejaculation that always or nearly always occurs prior to or within about 1 min of vaginal penetration from the first sexual experience (LPE) or a clinically significant and bothersome reduction in latency time, often to about 3 min or less (APE); [2] the inability to delay ejaculation on all or nearly all vaginal penetrations; and [3] negative personal consequences, such as distress, bother, frustration, and/or the avoidance of sexual intimacy. [3]. It has been claimed that the prevalence rate of LPE is 5% globally and 3% in China [5]. Current PE treatment strategies include behavioral therapy, selective serotonin reuptake inhibitors and selective phosphodiesterase inhibitors [6]. Among them, drug therapy is the first-line treatment for PE [7]. Classical selective serotonin reuptake inhibitors (SSRIs), such as dapoxetine, citalopram and Fortacin™, effectively delay ejaculation in patients with lifelong PE [8,9,10]. Most PE treatments are either experimental or used off-label [6]. Therefore, finding its effective biomarkers is a new approach to treat PE.

Previous twin and familial studies have demonstrated a genetic susceptibility to LPE, with genetic factors accounting for 30% of twins [11, 12]. However, the genetic variations in which genes affect LPE susceptibility have not been elucidated [13]. Among them, the research of 5-hydroxytryptamine (5-HT) and its related regulatory genes has a very sufficient theoretical basis [14]. 5-HT is the most important neurotransmitter and has been found to regulate ejaculation [15]. Tryptophan hydroxylase (TPH) is an important enzyme for 5-HT synthesis, and its expression level directly influences the synthesis amount of 5-HT, which in turn affects the function of 5-HT [16]. As one of the subtypes of TPH, TPH2 is specifically expressed in the raphe nucleus of 5-HT neurons, and regulates central 5-HT synthesis [17]. Studies have found that TPH2 SNV019 and rs4290270 are significantly associated with LPE in the Han population [18]. Besides, 5-HT needs to bind to its receptors to exert its biological effects. Among these receptors, HTR1A (5-hydroxytryptamine receptor 1 A) is one of the 5-HT receptors and plays an indispensable role in the regulation of ejaculation [19]. Another study has confirmed that the objective diagnostic indicators of LPE, including leptin and folic acid, can participate in the regulation of the 5-HT regulatory system by affecting the metabolism of 5-HT, and the number and function of receptors and transporters [20, 21].

In general, 5-HT concentration and abnormal receptors are important causes of PE. Therefore, we speculated that the 5-HT synthesis rate-limiting enzyme (TPH2) and HTR1A may contribute to the development of PE. So far, there is a suggestive genome-wide association study (GWAS) of the association between 33 gene polymorphisms and LPE risk in Chinese Han males [22]. Moreover, there are many polymorphisms of TPH2 and HTR1A genes, and most of them have been reported to be related to the occurrence of neurological diseases [23]. However, the effects of TPH2 and HTR1A gene polymorphisms on LPE are rarely studied in the Chinese Han population. Hence, we further explored the mechanism by which TPH2 and HTR1A gene polymorphisms affect LPE susceptibility in the Chinese Han population based on a case-control study. In addition, statistical analyses of LPE risk based on the levels of leptin, 5-HT, and folic acid were performed to identify potential risk factors for LPE. Our findings will provide a theoretical basis for further understanding of the pathogenesis of LPE.

Materials and methods

Study design

In this study, 91 patients with LPE and 362 normal controls aged 18 to 64 years were enrolled in the male urology department of Hainan General Hospital in China from January 2016 to December 2018. The SNPs in HTR1A and TPH2, which are related to 5-HT regulation, were selected as indexes to genotype the collected blood samples of participants. Logistic regression was used to analyze the correlation between SNPs of HTR1A and TPH2 and EP susceptibility, as well as the relationship with leptin, 5-HT and folic acid levels.

Ethical approval

This study was approved by the Ethics Committee of Hainan General Hospital and was conducted strictly in accordance with the World Medical Association Declaration of Helsinki. All participants signed an informed consent form after fully understanding the research purpose and protocol.

Study participants

All participants were Han Chinese males with permanent sexual partners. The age, leptin, 5-HT and folic acid levels, premature ejaculation diagnostic tool (PEDT), intravaginal ejaculatory latency time (IELT) and international erectile function scale (IIEF-5) scores of the participants were analyzed. The case group met the following criteria: (1) PE time definition is less than 1 min for LPE, and less than 3 min for secondary PE; (2) the above symptoms that last longer than 6 months [24]. The control group met the following conditions: 1) the lasts more than 3 min for LPE from the first sexual life. The case and control groups excluded those who met the following criteria: (1) men with APE; (2) men with anatomical deformities of the genitals that severely impair sexual function; (3) other abnormalities of sexual function (such as erectile dysfunction); (4) men with severe psychological disorders which cannot be well controlled by treatment; (5) men with other diseases, such as diabetes, stroke, myocardial infarction, cardiovascular disease, cancer, etc. At the same time, LPE patients were regularly reviewed and followed up.

SNPs selection and genotyping

Based on the 1,000 Genomes Project (http://www.1000genomes.org/) and dbSNP (https://www.ncbi.nlm.nih.gov/SNP/) database, three single nucleotide polymorphisms (SNPs) (rs878567, rs6294, and rs6295) in HTR1A and ten SNPs (rs11178996, rs11178997, rs11179001, rs10879346, rs1386492, rs11179023, rs7305115, rs11179041, rs10879352, and rs120074175) in TPH2 were selected. The minor allele frequencies (MAFs) of these candidate SNPs were greater than 5% in the global population.

Genomic DNA was extracted from peripheral blood samples of study participants by a Gold Mag-Mini Whole Blood Genomic DNA Purification kit (Gold Mag Co. Ltd., Xi’an, China) in strict accordance with the instructions [25]. NanoDrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA) was utilized to determine whether the concentration and purity of the extracted DNA meet the standards, and which can be used for further experiments [26]. The genotyping processes were performed on the Agena MassARRAY RS1000 (Shanghai, China) platform, and the SNP genotyping data were processed and analyzed by Agena Typer 4.0 software (version 4.0, Agena Bioscience, San Diego, CA, USA).

Statistical analyses

The experimental data were statistically analyzed by Microsoft Excel, SPSS 20.0 (SPSS, Chicago, IL), and PLINK software (version 1.07) (http://pngu.mgh.harvard.edu/purcell/plink/). The Chi-square test was used to evaluate whether the distribution of polymorphisms in the control group meets Hardy-Weinberg equilibrium (HWE). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to assess the correlation between SNPs and the risk of LPE based on logistic regression models. Linkage disequilibrium (LD) analysis and haplotype construction were performed by the Haploview software package (version 4.2) (https://www.broadinstitute.org/haploview/haploview), and the correlation between haplotypes and LPE susceptibility was examined using PLINK software [25].

Results

Characteristics of participants

In this study, the baseline data of 453 participants (91 LPE patients and 362 healthy people) including age and some clinical indexes were collected (Table 1). Age, PEDT, IELT, IIEF-5 score and 5-HT level were statistically significant between cases and controls (p < 0.001), and leptin and folic acid expression was not obvious different between cases and controls. The basic information of candidate SNPs included in this study is presented in Table 2. Except for TPH2-rs11179001 (p < 0.001), the other candidate SNPs in controls were in accordance with HWE (p > 0.01). Rs11178996 in TPH2 was related to a lower risk of LPE in the allelic model. Individuals carrying the “G” allele had a reduced risk of LPE (OR = 0.68, 95% CI = 0.49–0.96, p = 0.027) compared with those carrying the “A” allele.

Table 1 Comparison of basic characteristics between cases and healthy controls
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Table 2 HTR1A and TPH2 candidate SNPs and association with risk of LPE in allele model
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Relationship between TPH2 and HTR1A polymorphisms and LPE risk

The associations of different genotypes of TPH2 and HTR1A with LPE risk in multiple genetic models are shown in Table 3. The results showed that rs6295 in HTR1A was significantly associated with an increased risk of LPE in recessive model (“G/G” genotype: OR = 3.44, 95% CI = 1.03–11.54, p = 0.045) after adjustment. On the contrary, rs11178996 reduced the risk of LPE in codominant (“G/G” genotype: OR = 0.58, 95% CI = 0.35–0.98, p = 0.040), dominant (“A/G-G/G” genotype: OR = 0.58, 95% CI = 0.36–0.92, p = 0.020), and additive (OR = 0.71, 95% CI = 0.52–0.98, p = 0.039) models after adjustment.

Table 3 Logistic regression analysis of the association between HTR1A and TPH2 gene polymorphisms and risk of LPE.
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Relationship between TPH2 gene polymorphisms and LPE-related indicators

The relationship between TPH2 gene polymorphisms and the levels of leptin, 5-HT, and folic acid in LPE patients was also analyzed (Table 4). Rs11178997 in TPH2 was found to be correlated with leptin levels in codominant (p = 0.027) and dominant (p = 0.007) models. Rs10879346 in TPH2 showed a significant association with folic acid levels in the recessive model (p = 0.037). Rs1386492 in TPH2 showed a correlation with 5-HT levels in both codominant (p = 0.013) and recessive (p = 0.004) models. Rs11178996 in TPH2 was related to the levels of leptin (p = 0.031) and folic acid (p = 0.032) in the dominant model.

Table 4 Relationship between candidate gene polymorphism and leptin, 5-HT, and folic acid levels in LPE patients
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Relationship between haplotypes and LPE risk

Additionally, LD association analysis and haplotype construction of polymorphisms in HTR1A and TPH2 were also carried out. As shown in Fig. 1, rs878567 and rs6294 in HTR1A have a close chain relationship and constitute a haplotype block.

Fig. 1
figure 1

Haplotype block map of HTR1A SNPs. Block 1 includes rs878567 and rs6294 with D’ = 1 for the corresponding variants

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As shown in Fig. 2, rs10879346-rs1386492, rs11179023-rs7305115 and rs11179041-rs10879352 in TPH2 have the close chain relationship and constitute three haplotype blocks.

Fig. 2
figure 2

Haplotype block map of TPH2 SNPs. Block 1 includes rs10879346 and rs1386492 with D’ = 1 for the corresponding variants. Block 2 includes rs11179023 and rs7305115 with D’ = 1 for the corresponding variants. Block 3 includes rs11179041 and rs10879352 with D’ = 1 for the corresponding variants

Full size image

Moreover, the haplotype TPH2 Grs11179041Trs10879352 significantly reduced the risk of LPE after adjustment (OR = 0.44, 95% CI = 0.22–0.90, p = 0.024) (Table 5). The other haplotypes showed no significant correlation with LPE risk.

Table 5 HTR1A and TPH2 haplotype frequencies and the association with LPE risk
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Discussion

Studies have shown that genetic factors play an important role in LPE [11]. In our previous study, 13 genes (LACTBL1, SSBP3 and ACOT11) were found to be significantly associated with LPE risk in Chinese male Han population by genome-wide association analysis [22]. It was also reported that the genetic variation of 5-HT1B rs11568817 and 5-HT2C rs518147 were significantly associated with the occurrence of PE [27]. In this study, we investigated whether TPH2 and HTR1A gene polymorphisms in the 5-HT regulatory system are potentially associated with LPE susceptibility. Our results further confirmed that rs6295 in HTR1A was associated with an increased risk of LPE, while rs11178996 in TPH2 was related to a reduced risk of LPE. Different genotypes of rs11178997, rs10879346, and rs1386492 in the TPH2 gene were significantly correlated with the levels of leptin, folic acid, and 5-HT, respectively. Different genotypes of rs11178996 in the TPH2 gene were related to the levels of leptin and folic acid. The haplotype Grs11179041Trs10879352 showed an association with a decreased risk of LPE. This study preliminarily evaluated the effects of TPH2 and HTR1A gene polymorphisms on the susceptibility to LPE in the Chinese Han population.

The TPH2 gene is located on chromosome 12q21.1 and includes 11 exons [28]. The protein encoded by TPH2 catalyzes the first and rate-limiting steps in the biosynthesis of serotonin which is an important hormone and neurotransmitter. Currently, TPH2 gene polymorphisms have been extensively studied as potential predisposing factors for mental illnesses such as major affective disorder [29, 30], and major depressive disorder [28, 31]. Studies have reported that rs11178997, rs10879346, rs11179023, rs7305115, and rs120074175 in the TPH2 gene are all closely related to the occurrence of depression [31, 32]. The allele A of rs11178997 affects the expression of TPH2 by inhibiting its transcriptional activity in neurons, resulting in reduced 5-HT synthesis and depression [33]. The functional polymorphism rs120074175, in which arginine at position 441 in the coding region of the human gene is replaced by histidine, can cause 80% loss of TPH2 function, which in turn reduces 5-HT synthesis and triggers depression [34]. It is remarkable that our study explored the correlation between TPH2 polymorphisms and the risk of LPE. Though we did not find any significant correlation between rs11178997, rs10879346, rs11179023, rs7305115, and rs120074175 and the risk of LPE, we discovered that different genotypes of rs11178997, rs10879346, and rs1386492 were significantly related to the levels of leptin, folic acid, and 5-HT, respectively. Moreover, different genotypes of rs11178996 were associated with the levels of leptin and folic acid. Most importantly, there has been no research reported on rs11178996. Our study demonstrated that rs11178996 was correlated with decreased risk of LPE.

The HTR1A gene is located on chromosome 5q11.2-q13, encodes G protein-coupled receptors for 5-HT (serotonin), and belongs to the 5-HT receptor subfamily [35, 36]. The main function of HTR1A is to regulate the release of serotonin and the metabolism of dopamine and serotonin. It has been reported that HTR1A affects the occurrence of several diseases including periodic fever, menstrual cycle-dependence febrile episode [37], generalized anxiety disorder [38], and schizophrenia [39]. Previous studies have suggested that HTR1A polymorphisms are associated with various mental diseases, such as major depressive disorder [40], obsessive-compulsive disorder [41], and anxiety disorder [42]. There are few studies on the relationship between HTR1A polymorphisms and LPE. Until 2014, Janssen et al. [23] have revealed for the first time that HTR1A-rs6295 is related to the length of IELT in patients with LPE in the Dutch Caucasian population. Recently, Roaiah et al. [43] have explored the potential relationship between HTR1A-rs6295 and LPE risk in Egyptians, and have found that the genotype of patients with LPE is mostly “CG”, while the genotype of the controls is “GG”. Our study is the first to investigate the impact of HTR1A rs878567, rs6294, and rs6295 on the risk of LPE in the Chinese Han population. Our results indicated that the genotype of patients with LPE was mostly “CC”, which is consistent with the study reported by Janssen et al. [23] in the Dutch population, while is contrary to the study by Mohamed Farid Roaiah et al. [43] in the Egyptian population. This may be caused by genetic differences among people of different races. Although we did not discover any association of rs878567 and rs6294 with the risk of LPE, we noticed that rs6295 was correlated with an increased risk of LPE. Individuals carrying the “GG” genotype had a 3.44-fold increased risk of LPE compared with those carrying the “C/C-C/G” genotype. Rs6295 is located in the promoter region of the 5-HT1A gene, and can regulate the region-specific modification of HTR1A expression and transcription. Some researchers have claimed that the rs6295-G allele can bind to nuclear transcription factors, enhance the expression of 5-HT1A autoreceptors, and reduce the release of 5-HT to affect the risk of LPE [44, 45]. Therefore, we speculated that the genotype “GG” of rs6295 may also influence 5-HT metabolism by regulating the expression level of HTR1A, and thus it was related to the risk of LPE.

In this study, we explored the correlation between HTR1A and TPH2 gene polymorphisms and LPE susceptibility in Chinese Han males, and found that HTR1a-rs6295 increased the LPE risk in recessive models, while TPH2-rs11178996 was a protective factor for LPE occurrence. In addition, TPH2 SNPs were associated with leptin, 5-HT, and folic acid levels, and haplotype Grs11179041Trs10879352 showed a reduced risk of LPE. This study further demonstrated that genetic variation plays an important role in the occurrence and development of LPE, which is related to the expression of leptin, 5-HT and folic acid. Furthermore, it provides the basis and guidance for the research on the mechanism of genes and genotypes regulating the occurrence, development and treatment of LPE. Simultaneously, it also excavates new biomarkers and provides theoretical support for the clinical diagnosis, prevention and personalized treatment of LPE.

Our current study also has some limitations due to the small sample size, limited information on the clinical indicators of LPE, and the single study population which is only the Chinese Han population. Therefore, it is necessary to expand the sample size and carefully design high-quality studies to further validate our findings. At the same time, the anthropometric variables, nutritional status, duration of sexual relationships, total physical activity, smoking, alcohol consumption, drinking, psychological status and any drugs history of the participants in this study were missing not available for statistical analysis, and confounding factors should be further excluded in later studies. In addition, our samples only represent the Chinese Han population. Due to the genetic differences among different ethnic groups, we will collect more samples from various populations to verify the correlation between TPH2 and HTR1A gene polymorphisms and LPE risk.

Conclusion

To sum up, our study provided powerful evidence that HTR1A-rs6295 and TPH2-rs11178996 were significantly associated with the risk of LPE, and the haplotype Grs11179041Trs10879352 shows a decreased risk of LPE in the Chinese Han population. Studies have suggested that TPH2 and HTR1A polymorphisms may play a potential role in the development of LPE, which provides data support for the prevention, diagnosis and personalized treatment of LPE.

Data Availability

The datasets generated and/or analysed during the current study are available in the zenodo repository (https://www.zenodo.org/record/7726589#.ZA6W-flDSUk).

References

  1. Martin C, Nolen H, Podolnick J, Wang R. Current and emerging therapies in premature ejaculation: where we are coming from, where we are going. Int J urology: official J Japanese Urol Association. 2017;24(1):40–50.

    Article  Google Scholar 

  2. Gillman N, Gillman M. Premature ejaculation: aetiology and treatment strategies. Med Sci (Basel, Switzerland). 2019;7(11):102.

  3. Serefoglu EC, McMahon CG, Waldinger MD, Althof SE, Shindel A, Adaikan G, et al. An evidence-based unified definition of lifelong and acquired premature ejaculation: report of the second International Society for sexual Medicine Ad Hoc Committee for the definition of premature ejaculation. J Sex Med. 2014;11(6):1423–41.

    Article  PubMed  Google Scholar 

  4. Serefoglu EC, Cimen HI, Atmaca AF, Balbay MD. The distribution of patients who seek treatment for the complaint of ejaculating prematurely according to the four premature ejaculation syndromes. J Sex Med. 2010;7(2 Pt 1):810–5.

    Article  PubMed  Google Scholar 

  5. Althof SE, McMahon CG, Waldinger MD, Serefoglu EC, Shindel AW, Adaikan PG, et al. An update of the International Society of sexual Medicine’s guidelines for the diagnosis and treatment of premature ejaculation (PE). J Sex Med. 2014;11(6):1392–422.

    Article  PubMed  Google Scholar 

  6. Saleh R, Majzoub A, Abu El-Hamd M. An update on the treatment of premature ejaculation: a systematic review. Arab J Urol. 2021;19(3):281–302.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Salonia A, Bettocchi C, Boeri L, Capogrosso P, Carvalho J, Cilesiz NC, et al. European Association of Urology Guidelines on sexual and Reproductive Health-2021 update: male sexual dysfunction. Eur Urol. 2021;80(3):333–57.

    Article  PubMed  Google Scholar 

  8. Waldinger MD. Drug treatment options for premature ejaculation. Expert Opin Pharmacother. 2018;19(10):1077–85.

    Article  CAS  PubMed  Google Scholar 

  9. Rezakhaniha BJBBRA. Comparative study of Therapeutic Effects of two Medicinal Procedures of Citalopram in premature ejaculation. Biosci Biotechnol Res Asia. 2014;11(2):953–8.

    Article  Google Scholar 

  10. Bijan R, Soheila SJIJoRM. Efficacy of selective serotonin reuptake inhibitor (SSRI) in patient with premature ejaculation. IJRM. 2010;8(2):55–9.

    Google Scholar 

  11. Jern P, Santtila P, Johansson A, Varjonen M, Witting K, von der Pahlen B, et al. Evidence for a genetic etiology to ejaculatory dysfunction. Int J Impot Res. 2009;21(1):62–7.

    Article  CAS  PubMed  Google Scholar 

  12. Jern P, Santtila P, Witting K, Alanko K, Harlaar N, Johansson A, et al. Premature and delayed ejaculation: genetic and environmental effects in a population-based sample of finnish twins. J Sex Med. 2007;4(6):1739–49.

    Article  PubMed  Google Scholar 

  13. Wang F, Luo D, Chen J, Pan C, Wang Z, Fu H et al. Effects of CYP24A1 polymorphisms on premature ejaculation: a case-control study. J Gen. 2022;101:43.

  14. Huang Y, Zhang X, Gao J, Tang D, Gao P, Peng D, et al. Association of STin2 VNTR polymorphism of serotonin transporter gene with lifelong premature ejaculation: a case-control study in Han chinese subjects. Med Sci monitor: Int Med J experimental Clin Res. 2016;22:3588–94.

    Article  CAS  Google Scholar 

  15. Zhu T, Gao P, Gao J, Liu X, Jiang H, Zhang X. The upregulation of tryptophan hydroxylase-2 expression is important for premature ejaculation treatment with the selective serotonin reuptake inhibitor. Andrology. 2022;10(3):595–603.

    Article  CAS  PubMed  Google Scholar 

  16. Mosienko V, Beis D, Pasqualetti M, Waider J, Matthes S, Qadri F, et al. Life without brain serotonin: reevaluation of serotonin function with mice deficient in brain serotonin synthesis. Behav Brain Res. 2015;277:78–88.

    Article  CAS  PubMed  Google Scholar 

  17. Matthes S, Mosienko V, Popova E, Rivalan M, Bader M, Alenina N. Targeted Manipulation of Brain Serotonin: RNAi-Mediated Knockdown of Tryptophan Hydroxylase 2 in Rats. 2019;10(7):3207–17.

  18. Fu X, Zhang X, Jiang T, Huang Y, Cheng P, Tang D, et al. Association between lifelong premature ejaculation and polymorphism of Tryptophan hydroxylase 2 gene in the Han Population. Sex Med. 2020;8(2):223–9.

    Article  PubMed  PubMed Central  Google Scholar 

  19. de Jong TR, Neumann ID. Moderate role of oxytocin in the pro-ejaculatory effect of the 5-HT1A receptor agonist 8-OH-DPAT. J Sex Med. 2015;12(1):17–28.

    Article  PubMed  Google Scholar 

  20. Wang B, Yang C, Tang K. Serum leptin and 5-hydroxytryptamine measurements for the diagnosis and treatment of premature ejaculation. Urology. 2013;82(6):1336–40.

    Article  PubMed  Google Scholar 

  21. Cai T, Verze P, Massenio P, Tiscione D, Malossini G, Cormio L, et al. Rhodiola rosea, folic acid, zinc and biotin (EndEP(®)) is able to improve ejaculatory control in patients affected by lifelong premature ejaculation: results from a phase I-II study. Experimental and therapeutic medicine. 2016;12(4):2083–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Wang F, Luo D, Chen J, Pan C, Wang Z, Fu H, et al. Genome-wide Association analysis to search for new loci Associated with lifelong premature ejaculation risk in Chinese Male Han Population. world J men’s health. 2022;40(2):330–9.

    Article  CAS  Google Scholar 

  23. Janssen PK, van Schaik R, Zwinderman AH, Olivier B, Waldinger MD. The 5-HT1A receptor C(1019)G polymorphism influences the intravaginal ejaculation latency time in dutch caucasian men with lifelong premature ejaculation. Pharmacol Biochem Behav. 2014;121:184–8.

  24. Kam SC, Han DH, Lee SW. The diagnostic value of the premature ejaculation diagnostic tool and its association with intravaginal ejaculatory latency time. J Sex Med. 2011;8(3):865–71.

    Article  PubMed  Google Scholar 

  25. Jin TB, Ren Y, Shi X, Jiri M, He N, Feng T, et al. Genetic variations in the CLNK gene and ZNF518B gene are associated with gout in case-control sample sets. Rheumatol Int. 2015;35(7):1141–7.

    Article  CAS  PubMed  Google Scholar 

  26. Wang N, Wang L, Yang H, Zhang HQ, Lan B, He X, et al. Multiple genetic variants are associated with colorectal cancer risk in the Han Chinese population. Eur J cancer prevention: official J Eur Cancer Prev Organisation (ECP). 2015;24(1):1–5.

    Article  Google Scholar 

  27. Sonkurt MD, Güleç G, Coşan DT, Çalış İU, Mutlu F, Üre İ, et al. Single nucleotide polymorphisms in 5-HT receptors in the etiology of premature ejaculation. Revista Int de andrologia. 2022;20(4):217–24.

    Google Scholar 

  28. Gao J, Pan Z, Jiao Z, Li F, Zhao G, Wei Q, et al. TPH2 gene polymorphisms and major depression–a meta-analysis. PLoS ONE. 2012;7(5):e36721.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Mandelli L, Antypa N, Nearchou FA, Vaiopoulos C, Stefanis CN, Serretti A, et al. The role of serotonergic genes and environmental stress on the development of depressive symptoms and neuroticism. J Affect Disord. 2012;142(1–3):82–9.

    Article  CAS  PubMed  Google Scholar 

  30. Cichon S, Winge I, Mattheisen M, Georgi A, Karpushova A, Freudenberg J, et al. Brain-specific tryptophan hydroxylase 2 (TPH2): a functional Pro206Ser substitution and variation in the 5’-region are associated with bipolar affective disorder. Hum Mol Genet. 2008;17(1):87–97.

    Article  CAS  PubMed  Google Scholar 

  31. Nazree NE, Loke AC, Zainal NZ, Mohamed Z. Lack of association between TPH2 gene polymorphisms with major depressive disorder in multiethnic malaysian population. Asia-Pacific psychiatry: official journal of the Pacific Rim College of Psychiatrists. 2015;7(1):72–7.

    Article  PubMed  Google Scholar 

  32. Yang J, Zhao X, Ma J, Qiao Z, Yang X, Zhao E, et al. The Interaction of TPH2 and 5-HT2A polymorphisms on major depressive disorder susceptibility in a chinese Han Population: a case-control study. Front Psychiatry. 2019;10:172.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Scheuch K, Lautenschlager M, Grohmann M, Stahlberg S, Kirchheiner J, Zill P, et al. Characterization of a functional promoter polymorphism of the human tryptophan hydroxylase 2 gene in serotonergic raphe neurons. Biol Psychiatry. 2007;62(11):1288–94.

    Article  CAS  PubMed  Google Scholar 

  34. Van Den Bogaert A, De Zutter S, Heyrman L, Mendlewicz J, Adolfsson R, Van Broeckhoven C et al. Response to Zhang (2005): loss-of-function mutation in tryptophan hydroxylase-2 identified in unipolar major Depression. Neuron 45, 11–16. Neuron. 2005;48(5):704; author reply 5–6.

  35. Lin H, Lei Y, Zhang B, Dai Z, Lu X. Common variants of HTR1A and SLC6A4 confer the increasing risk of Schizophrenia susceptibility: a population-based association and epistasis analysis. Am J Med Genet Part B Neuropsychiatric genetics: official publication Int Soc Psychiatric Genet. 2015;168(8):749–55.

    Article  CAS  Google Scholar 

  36. Huang YY, Battistuzzi C, Oquendo MA, Harkavy-Friedman J, Greenhill L, Zalsman G, et al. Human 5-HT1A receptor C(-1019)G polymorphism and psychopathology. Int J Neuropsychopharmacol. 2004;7(4):441–51.

    Article  CAS  PubMed  Google Scholar 

  37. Jiang YC, Wu HM, Cheng KH, Sunny Sun H. Menstrual cycle-dependent febrile episode mediated by sequence-specific repression of poly(ADP-ribose) polymerase-1 on the transcription of the human serotonin receptor 1A gene. Hum Mutat. 2012;33(1):209–17.

    Article  CAS  PubMed  Google Scholar 

  38. Cutler NR, Hesselink JM, Sramek JJ. A phase II multicenter dose-finding, efficacy and safety trial of ipsapirone in outpatients with generalized anxiety disorder. Prog Neuro-psychopharmacol Biol Psychiatry. 1994;18(3):447–63.

    Article  CAS  Google Scholar 

  39. Guan F, Lin H, Chen G, Li L, Chen T, Liu X, et al. Evaluation of association of common variants in HTR1A and HTR5A with schizophrenia and executive function. Sci Rep. 2016;6:38048.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Zhang R, Bi Y, Niu W, Huang X, Chen S, Li X, et al. Association study of 5-HT1A, 5-HT2A polymorphisms with schizophrenia and major depressive disorder in the Han Chinese population. Neurosci Lett. 2016;635:39–43.

    Article  CAS  PubMed  Google Scholar 

  41. Alizadeh N, Nosrat N, Jahani Z, Ahmadiani A. Association of HTR1A gene polymorphisms with obsessive-compulsive disorder and its treatment response: the influence of sex and clinical characteristics. 2019;129(3):264–72.

  42. Huang JH, Chang HA, Fang WH, Ho PS, Liu YP, Wan FJ et al. Serotonin receptor 1A promoter polymorphism, rs6295, modulates human anxiety levels via altering parasympathetic nervous activity. 2018;137(3):263–72.

  43. Roaiah MF, Elkhayat YI, Rashed LA, GamalEl Din SF, El Guindi AM, Soliman IF, et al. 5HT-1A receptor polymorphism effects ejaculatory function in egyptian patients with lifelong premature ejaculation. Revista Int de andrologia. 2019;17(4):138–42.

    Google Scholar 

  44. Sener EF, Cıkılı Uytun M, Korkmaz Bayramov K, Zararsiz G, Oztop DB, Canatan H, et al. The roles of CC2D1A and HTR1A gene expressions in autism spectrum disorders. Metab Brain Dis. 2016;31(3):613–9.

    Article  CAS  PubMed  Google Scholar 

  45. Albert PR, Lemonde S. 5-HT1A receptors, gene repression, and depression: guilt by association. The Neuroscientist: a review journal bringing neurobiology neurology and psychiatry. 2004;10(6):575–93.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Hainan General Hospital for providing blood samples and all participants in this study.

Funding

This study was funded by the National Natural Science Foundation of China: Project of Regional Science Foundation (No. 81560250).

Author information

Author notes

  1. Fei Wang and Defan Luo are first authors.

Authors and Affiliations

  1. Department of Urology, Hainan General Hospital, Affiliated Hainan Hospital of Hainan Medical University, No.19, Xiuhua Road, Xiuying District, Haikou, Hainan Province, 570311, China

    Fei Wang, Cuiqing Pan, Zhongyao Wang, Housheng Fu, Jianbing Xu & Weifu Wang

  2. Department of Lung Transplatation, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan, 571199, China

    Defan Luo

  3. Department of Urology, Affiliated Hospital of Xiangnan University, Chenzhou, Hunan, 423000, China

    Jianxiang Chen

  4. Ministry of Science and Education, Hainan Women and Children’s Medical Center, Haikou, Hainan, 571100, China

    Shaowei Mo

  5. Library, Hainan Medical University, Haikou, Hainan, 571199, China

    Liying Zhuang

  6. Department of Kidney Transplatation, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, 571199, China

    Meng Yang

Authors
  1. Fei Wang
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  11. Weifu Wang
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Contributions

Weifu Wang conceived and designed experiments; Defan Luo, Jianxiang Chen, and Cuiqing Pan performed experiments; Zhongyao Wang and Housheng Fu collected samples; Jianbing Xu and Meng Yang analyzed data; Shaowei Mo and Liying Zhuang contributed reagents/materials/analysis tools; Fei Wang and Defan Luo drafted and revised the paper.

Corresponding author

Correspondence to Weifu Wang.

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Human accordance

All experimental protocols followed the Declaration of Helsinki.

Informed consent to participate

Written informed consent was obtained from each participant before the research.

Study approval

The study was approved by the Ethics Committee of Hainan General Hospital.

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Not applicable.

Conflict of interest

The authors declared that they have no conflicts of interest.

Competing interests

The authors declare no competing interests.">

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Wang, F., Luo, D., Chen, J. et al. Influence of TPH2 and HTR1A polymorphisms on lifelong premature ejaculation risk among the chinese Han population. BMC Urol 23, 86 (2023). https://doi.org/10.1186/s12894-023-01222-9

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Keywords

BMC Urology

ISSN: 1471-2490

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